Correspondence of banding patterns of Giemsa C-band and DAPI/CMA fluorochromes of the same specimen of Aconitum sanyoense was examined. It appeared that there were various C-bands, that is, AT-rich (C⁺/DAPI⁺/CMA^-), GC-rich (C⁺/DAPI^-/CMA⁺), and intermediate (C⁺/DAPI^-/CMA^-) C-bands. Furthermore, some so-called euchromatic segments showed AT-rich DNA sequences (C^-/DAPI⁺/CMA^-). Determination of the combination of these banding patterns may advance detailed detection of cytogenetical variation among individuals of this species.